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Item #:
220513550
TG Electrophoresis Buffer (10X), Tris-Glycine Transfer Buffer for Western Blots and Native Gels, pH 8.3, 500mL/Bottle, Single Bottle
Item #:
220513550
MMK 78767
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Provide stable acid-base environment for relevant molecular biology experiments to ensure the stability of the structure and function of biomolecules.
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Product Details
| Brand Name | CryoKing |
| Manufacturer | BIOLOGIX |
| Part Number | TG-510-1 |
| Unit Count | 1.0 Count |
| Included Components | TG Electrophoresis Buffer |
| Brand | CryoKing |
| Item Weight | 1.1 lbs (500 grams) |
Product Description
TG Electrophoresis Buffer (10X), Tris-Glycine Transfer Buffer for Western Blots and Native Gels, pH 8.3, 500mL/Bottle, Single Bottle
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Statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition.
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Customer Questions & Answers
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Question:
What is the purpose of TG Electrophoresis Buffer (10X)?
Answer: TG Electrophoresis Buffer (10X) is primarily used to provide optimal conditions for protein separation during electrophoresis. It supports stable pH levels, critical for preserving protein integrity and separation efficiency. This buffer is particularly effective in preparing samples for Western blots and native gels, ensuring clear protein bands. In laboratory settings, using the right buffer can significantly impact the clarity and accuracy of results, helping researchers achieve reliable data in protein studies. -
Question:
What are the key components of the Tris-Glycine Transfer Buffer?
Answer: The Tris-Glycine Transfer Buffer consists of Tris base and glycine in a specific pH 8.3 formulation. Tris acts as a buffering agent, maintaining a stable environment for proteins, while glycine facilitates the transfer process during the electroblotting phase. This combination is essential for achieving efficient protein migration, making it suitable for a variety of protein analysis techniques. Researchers often choose this buffer for its effectiveness in achieving high-quality protein transfers in Western blotting applications. -
Question:
What types of gels can I use with this buffer?
Answer: TG Electrophoresis Buffer (10X) is versatile and can be used with both polyacrylamide and native agarose gels, allowing for a range of applications in protein analysis. Polyacrylamide gels are typically used for separating proteins based on molecular weight, while native gels maintain protein conformations, making them suitable for assessing enzymatic activity or binding properties. This flexibility makes the buffer ideal for various research requirements, whether you're analyzing purified proteins or conducting complex biochemical assays. -
Question:
How should I prepare the TG Electrophoresis Buffer for use?
Answer: To prepare TG Electrophoresis Buffer from the 10X concentration, you need to dilute it to a 1X working solution. Mix one part of the 10X buffer with nine parts of distilled water. Ensure the final concentration maintains the ideal pH of 8.3 for optimal performance. This step is crucial as the correct dilution ensures efficient protein separation and transfer during experiments. Many researchers prepare their buffer solutions fresh before experiments for best results in their protein analyses. -
Question:
Is TG Electrophoresis Buffer compatible with all protein types?
Answer: TG Electrophoresis Buffer is compatible with a wide variety of proteins; however, certain proteins may require specific considerations regarding their size and charge. The buffer supports a stable environment ideal for most recombinant and native proteins. It's particularly effective for small to medium-sized proteins. For proteins with unique properties or extreme isoelectric points, adjustments may be necessary to optimize separation outcomes. Researchers often perform preliminary tests to determine compatibility for specific applications. -
Question:
Can I store TG Electrophoresis Buffer after opening?
Answer: Yes, you can store TG Electrophoresis Buffer after opening. It is recommended to keep it tightly sealed and stored in a cool, dark place to prevent degradation of its active components. Ensure it is labeled with the date opened. Proper storage helps maintain buffer efficacy over time, ensuring reliable results during experiments. Many laboratories opt to store their buffers in dedicated coolers to ensure they remain in optimal condition for future use. -
Question:
What safety precautions should I take when using this buffer?
Answer: When using TG Electrophoresis Buffer, it’s important to wear appropriate personal protective equipment, such as gloves and safety goggles, to avoid skin and eye contact. While the buffer is not classified as hazardous, handling chemicals requires caution. Always work in a well-ventilated area and follow your institution’s safety protocols. This awareness reduces the risk of exposure and ensures a safe working environment during protein analyses. -
Question:
How does the pH affect the performance of TG Electrophoresis Buffer?
Answer: The pH of TG Electrophoresis Buffer, optimized at 8.3, plays a critical role in buffering capacity and protein stability during electrophoresis. An ideal pH ensures proteins maintain their native structures and do not denature, which is essential during the separation process. Deviating from this pH can lead to altered migration patterns and compromised results. Researchers consistently monitor pH to ensure accuracy in their experiments, as even slight variations can notably impact protein behavior. -
Question:
Where can I buy TG Electrophoresis Buffer in Myanmar?
Answer: You can buy TG Electrophoresis Buffer at Ubuy in Myanmar. Ubuy offers a convenient online platform where you can access this product along with various laboratory supplies. Shopping through Ubuy helps you find essential research materials with ease, ensuring that your labs are well-equipped for your experiments. With user-friendly navigation and reliable customer support, getting your scientific resources becomes more straightforward.
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MMK 78767
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Features & Benefits
- Stable acid-base environment ensures biomolecule reliability.
- Ready-to-use formulation saves preparation time.
- Excellent sealing prevents reagent leakage and impurities.
- Versatile applications for various molecular biology techniques.
- Consistent quality minimizes experimental errors.
- High-concentration formula reduces storage needs.
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